Tracking chromatin states using controlled DNase I treatment and real-time PCR

R Martins, A Platts, S Krawetz - Cellular and Molecular Biology …, 2007 - degruyter.com
Cellular and Molecular Biology Letters, 2007degruyter.com
A novel approach to DNase I-sensitivity analysis was applied to examining genes of the
spermatogenic pathway, reflective of the substantial morphological and genomic changes
that occur during this program of differentiation. A new real-time PCR-based strategy that
considers the nuances of response to nuclease treatment was used to assess the nuclease
susceptibility through differentiation. Data analysis was automated with the K-Lab PCR
algorithm, facilitating the rapid analysis of multiple samples while eliminating the subjectivity …
Abstract
A novel approach to DNase I-sensitivity analysis was applied to examining genes of the spermatogenic pathway, reflective of the substantial morphological and genomic changes that occur during this program of differentiation. A new real-time PCR-based strategy that considers the nuances of response to nuclease treatment was used to assess the nuclease susceptibility through differentiation. Data analysis was automated with the K-Lab PCR algorithm, facilitating the rapid analysis of multiple samples while eliminating the subjectivity usually associated with Ct analyses. The utility of this assay and analytical paradigm as applied to nuclease-sensitivity mapping is presented.
De Gruyter
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